M7802

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse

~2 mg/mL, clone ERK-PT115, purified immunoglobulin, buffered aqueous solution

• Sinonimo/i: Anti-ERK, Anti-ERK-2, Anti-ERK2, Anti-ERT1, Anti-MAPK2, Anti-NS13, Anti-P42MAPK, Anti-PRKM1, Anti-PRKM2, Anti-p38, Anti-p40, Anti-p41, Anti-p41mapk, Anti-p42-MAPK
• Numero MDL: MFCD01633612
• Codice UNSPSC: 12352203
• NACRES: NA.44

Proprietà

• Origine biologica: mouse
• Coniugato: unconjugated
• Forma dell’anticorpo: purified immunoglobulin
• Tipo di anticorpo: primary antibodies
• Clone: ERK-PT115, monoclonal
• Forma fisica: buffered aqueous solution
• PM: antigen, ERK-1 44 kDa; antigen, ERK-2 42 kDa
• Reattività contro le specie: rat, human
• Concentrazione: ~2 mg/mL
• tecniche: capture ELISA: suitable; immunocytochemistry: suitable; microarray: suitable; western blot: 0.5 μg/mL using extract of a rat fibroblast cell line, Rat1, activated with sorbitol
• Isotipo: IgG1
• N° accesso UniProt: P28482
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... MAPK1(5594) , MAPK3(5595) ; rat ... Mapk1(116590) , Mapk3(50689)

Descrizione

Descrizione generale

Activated/ Monophosphorylated (Phosphothreonine ERK-1&2) (mouse IgG1 isotype) is derived from the ERK-PT115 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Mitogen-activated protein kinase 1 (MAPK1) is mapped to human chromosome 22q11.22 and MAPK3 is present on human chromosome 16p11.2.

Specificità

The antibody reacts specifically with the monophosphorylated threonine and the active diphosphorylated forms of MAP kinase (ERK-1 and ERK-2). It does not recognize the non-phosphorylated form of the MAP kinase molecule, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated threonine residue within the regulatory site of active MAP kinase (e.g.,Thr¹⁸³ in ERK-2).

Immunogeno

synthetic peptide HTGFLpTEYVAT, corresponding to the phosphorylated form of ERK-activation loop.

Applicazioni

Monoclonal Anti-MAP Kinase, Activated/monophosphorylated (Phosphothreonine ERK-1&2) antibody produced in mouse has been used in:

• immunoblotting
• western blotting
• immunofluorescence
• enzyme linked immunosorbent assay (ELISA)
• immunochemistry

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable