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Key Documents

M1919

Sigma-Aldrich

Monoclonal Anti-Macrophages antibody produced in mouse

clone LN-5, ascites fluid

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About This Item

Numero MDL:
Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

mouse

Coniugato

unconjugated

Forma dell’anticorpo

ascites fluid

Tipo di anticorpo

primary antibodies

Clone

LN-5, monoclonal

contiene

15 mM sodium azide

Reattività contro le specie

human

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using formalin-fixed, paraffin-embedded human tonsil sections

Isotipo

IgM

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

modifica post-traduzionali bersaglio

unmodified

Descrizione generale

Monoclonal Anti-Human Macrophages (mouse IgM isotype) is derived from the LN-5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Macrophages are widely distributed throughout the body. They belong to the mononuclear phagocytes family. Macrophages have abundant cytoplasm and are also termed histiocyte.

Immunogeno

deparaffinized human peripheral blood mononuclear cells

Azioni biochim/fisiol

Macrophages play a crucial role in body defense mechanisms and have phagocytic functionality. They are heterogeneous in morphology, cytochemistry, function and surface antigens. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and the diagnosis of various inflammation.

Stato fisico

The product is provided as ascites fluid with 0.1% sodium azide as a preservative.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month. For extended storage freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

nwg

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


Certificati d'analisi (COA)

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Slava Epelman et al.
Immunity, 41(1), 21-35 (2014-07-19)
Macrophages are distributed in tissues throughout the body and contribute to both homeostasis and disease. Recently, it has become evident that most adult tissue macrophages originate during embryonic development and not from circulating monocytes. Each tissue has its own composition
L Bhoopat et al.
Blood, 71(4), 1079-1085 (1988-04-01)
Two monoclonal antibodies (LN-4, LN-5) reactive to human macrophages in B5 formalin-fixed, paraffin-embedded tissue sections have been produced by using deparaffinized cell extracts of peripheral blood mononuclear cells. Both monoclonal antibodies were initially identified on paraffin-embedded sections of hyperplastic lymph
S S Shin et al.
Human pathology, 23(6), 686-694 (1992-06-01)
To provide baseline information on the immunoarchitecture of normal bone marrow, we studied cryostat-cut, frozen, and paraffin-embedded, fixed tissue sections prepared from 21 core biopsies of normal bone marrow obtained during bone marrow harvests for transplantation. A large panel of

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