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Key Documents

L2018

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli K-235

purified by gel-filtration chromatography

Sinonimo/i:

LPS

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About This Item

Numero CE:
Numero MDL:
Codice UNSPSC:
12352201
NACRES:
NA.25

Origine biologica

Escherichia coli (K-235)

Livello qualitativo

Forma fisica

lyophilized powder

Purificato mediante

gel-filtration chromatography

Impurezze

<1% Protein

Colore

white to yellow cast

Solubilità

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

Condizioni di spedizione

ambient

Temperatura di conservazione

2-8°C

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Descrizione generale

This product is extracted from E. coli serotype K235 and purified by gel filtration. The source strain is ATCC 13027.

Azioni biochim/fisiol

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Nota sulla preparazione

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Altre note

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Skull and crossbones

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 2 Oral

Codice della classe di stoccaggio

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

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