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Key Documents

HYSC010

Sigma-Aldrich

HyStem®-C Cell Culture Scaffold Kit

Trial Kit, For 2.5 mL of hydrogel scaffold solution

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About This Item

Codice UNSPSC:
12352207
NACRES:
NA.75

Livello qualitativo

Temperatura di conservazione

−20°C

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Applicazioni

HyStem®-C cell culture scaffold kit has been used to prepare HyStem hydrogels to culture breast cancer cells.

Caratteristiche e vantaggi

Customizable, well characterized and consistent, Hystem-C hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-C offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Synthetic - As a synthetic matrix, Hystem-C offers greater control of the cellular environment than ambiguous extracted ECM alternatives.  All components are well characterized and consistently formulated. Hystem-C contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), and Water.
  • Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-C hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.

Hystem-C enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

The Hystem-C Trial Kit enables researchers to evaluate the Hystem-C technology in their own hands and confirm that Hystem-C is the right choice for their research.

With Hystem-C, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-C has been successfully used to expand human embryonic stem cells, human mesenchymal stem cells, neural progenitor cells, and hepatic progenitor cells.
Customizable, well characterized and consistent, Hystem-C hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
  • Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-C offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
  • Synthetic - As a synthetic matrix, Hystem-C offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. Hystem-C contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), and Water.
  • Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-C hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.

Note legali

Hystem is a registered trademark of BIOTIME, INC

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Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Eye Dam. 1 - Skin Irrit. 2 - Skin Sens. 1

Codice della classe di stoccaggio

10 - Combustible liquids


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Independently Tuning the Biochemical and Mechanical Properties of 3D Hyaluronan-Based Hydrogels with Oxime and Diels?Alder Chemistry to Culture Breast Cancer Spheroids.
Baker A E, et al.
Biomacromolecules, 18(12), 4373-4384 (2017)

Articoli

Hyaluronan is a major component of the extracellular matrix. It’s the simplest glycosaminoglycan, and it plays several biological roles.

Heparin is distinct from heparan sulfate proteoglycans (HS) which is primarily produced by mast cells. While, HS is produced by all cell types. Know more about the difference between heparin and heparin sulfate proteoglycan and select the right component for your study.

Cell delivery vehicle are components that can be associated with cells and that allow transplantation in human or animals hosts. These can be natural or synthetic components that will form a gel, embedding the cells.

Hystem 3D hydrogels are based on hyaluronic acid, a major component of the extracellular matrix. 3D cell culture can be done using Hystem hydrogels.

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