HPA008038

ANTI-CDK12 antibody produced in rabbit

Prestige Antibodies^® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

• Sinonimo/i: Anti-CDC2-related protein kinase 7, Anti-CRKRS, Anti-Cdc2-related kinase, arginine/serine-rich, Anti-Cell division cycle 2-related protein kinase 7, Anti-CrkRS
• Codice UNSPSC: 12352203
• Numero Human Protein Atlas: HPA008038
• NACRES: NA.43

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 100
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Nome Commerciale: Prestige Antibodies^® Powered by Atlas Antibodies
• Forma fisica: buffered aqueous glycerol solution
• Reattività contro le specie: human
• tecniche: immunofluorescence: 0.25-2 μg/mL; immunohistochemistry: 1:50-1:200
• Sequenza immunogenica: GFSAIDTDERNSGPALTESLVQTLVKNRTFSGSLSHLGESSSYQGTGSVQFPGDQDLRFARVPLALHPVVGQPFLKAEGSSNSVVHAETKLQNYGELGPGTTGASSSGAGLHWGGPTQSSAY
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CRKRS(51755)

Descrizione

Descrizione generale

Cyclin-dependent kinase 12 (CDK12) is a member of the cyclin-dependent kinase (CDK) family of serine/threonine protein kinases. It is located at 17q12 on the human chromosome. CDK12 is composed of 21 arginine/serine-rich (RS) motifs, a proline-rich motif in between the RS motif, a central kinase domain and a C-terminal region. CDK12 gene encodes a C-terminal repeat domain (CTD) kinase that contains an arginine/serine-rich (RS) domain (CrkRS). It is a 1490 amino acid long and colocalizes with SC35 speckles.

Immunogeno

Cell division cycle 2-related protein kinase 7 recombinant protein epitope signature tag (PrEST)

Applicazioni

ANTI-CDK12 antibody produced in rabbit has been used in immunohistochemistry.

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies^® protocols and other useful information.

Azioni biochim/fisiol

Cyclin-dependent kinase 12 (CDK-12) interacts with cyclin L1 and cyclin L2 and regulates alternative splicing. This kinase phosphorylates the CTD of the large subunit of RNA polymerase II and regulates transcription elongation. It forms a link between transcription and splicing machinery. It negatively regulates MAPK pathway and has a role in the resistance to estrogen signaling inhibitors during endocrine therapy.

Cyclin-dependent kinase 12 (CDK12) is a transcription-associated CDK. It plays a role in DNA-damage response, splicing and differentiation. Overexpression and mutation of the CDK12 gene is associated with several malignancies.

Caratteristiche e vantaggi

Prestige Antibodies^® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies^® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:

• IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
• Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST71450

Stato fisico

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Note legali

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)