H8791

Transforming Growth Factor-β3 human

HumanKine^®, 95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Sinonimo/i: TGF-β3
• Numero MDL: MFCD00213054
• Codice UNSPSC: 12352202
• NACRES: NA.77

Proprietà

• Nome del prodotto: Transforming Growth Factor-β3 human, TGF-β3, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• Origine biologica: human
• Livello qualitativo: 100
• Ricombinante: expressed in HEK 293 cells
• Saggio: ≥95% (SDS-PAGE)
• Stato: lyophilized powder
• Potenza: ≤1.0 ng/mL EC₅₀
• Qualità: endotoxin tested
• PM: dimer 25 kDa (non-glycosylated)
• Confezionamento: pkg of 5 μg
• Produttore/marchio commerciale: HumanZyme
• Condizioni di stoccaggio: avoid repeated freeze/thaw cycles
• tecniche: cell culture | mammalian: suitable
• Impurezze: ≤1 EU/μg
• N° accesso UniProt: P10600
• Temperatura di conservazione: −20°C
• Informazioni sul gene: human ... TGFB3(7043)

Descrizione

Descrizione generale

TGF-β3 (transforming growth factor-β3) belongs to the TGF β superfamily. The TGFβ3 gene is mapped to human chromosome 14q24.3

Azioni biochim/fisiol

TGF-β3 (transforming growth factor-β3) regulates lymphocyte proliferation, apoptosis, hematopoiesis and embryogenesis to maintain immune homeostasis. TGF-β plays an important role in cell growth, differentiation, and survival. TGF-β3 specifically promotes chondrogenic differentiation.TGF-β3 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils. TGF-β3 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β isoforms is known to participate in wound healing and tissue fibrosis. TGF-β3 is crucial for tissue restoration and scarless tissue repair. Mutation in the TGFβ3 gene is associated with development of non-syndromic cleft palate only (NS CPO), a rare congenital disease.

TGF-β3 is less prevalent in natural expression than either TGF-β1 or TGF-β2, but it is the most abundant mRNA expressed in chick embryos. It is also expressed in human umbilical cord, in a variety of mesenchymal cells of human and rodent origin, and in several human carcinoma cells.

Stato fisico

Lyophilized from a 0.2 μm filtered solution of 50 mM NaOAc pH 4.5

Nota sulla preparazione

HumanKine TGF-β3 is expressed in human HEK 293 cells as a mature, disulfide linked, non- glycosylated homodimer with a predicted molecular mass of 25 kDa.
This product is lyophilized from a solution of 50mM NaOAc, pH 3.7.

Risultati analitici

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Note legali

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable