H8164

Anti-dimethyl, phospho-Histone H1.4 (diMe-Lys²⁶. pSer²⁷)

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-H1.4K26me2S27p, Anti-H1K26me2S27p
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen ~35 kDa
• Reattività contro le specie: human
• Concentrazione: ~1.5 mg/mL
• tecniche: western blot: 0.5-1 μg/mL using acid-extracted fraction of HL60 cells
• N° accesso UniProt: P10412
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: dimethylation (Lys26), phosphorylation (pSer27)
• Informazioni sul gene: human ... HIST1H1E(3008)

Descrizione

Specificità

4 (diMe-Lys26, pSer27) specifically recognizes human histone H1.

Immunogeno

dimethylated and phosphorylated synthetic peptide corresponding to amino acids 22-33 (diMe-Lys²⁶, pSer²⁷) of human histone H1.4.

Applicazioni

Anti-dimethyl, phospho-Histone H1.4 (diMe-Lys26, pSer27) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using acid-extracted fraction of HL60 cells.

Azioni biochim/fisiol

The building block of chromatin is the nucleosome which is comprised of an octamer of core histones (H2A, H2B, H3, and H4) around which 147 bp of DNA are wrapped. In mammalian cells, four histone H1 variants (H1.2 to H1.5) are present in all somatic cells, and a fifth (H1.1) is restricted to thymus, testis, and spleen and possibly lymphocytic and neuronal cells. Histone H1.4 is di-methylated or acetylated at Lys26. Lys26 is located within the flexible N-terminal domain of H1.4, just preceding the globular domain.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Stoccaggio e stabilità

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)