H0160
HIV-1 gag
≥95% (SDS-PAGE), recombinant, expressed in E. coli, Galactosidase-tagged
Sinonimo/i:
p24 HIV antigen
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About This Item
Prodotti consigliati
Ricombinante
expressed in E. coli
Livello qualitativo
Saggio
≥95% (SDS-PAGE)
PM
39 kDa (plus 114 kDa β-Gal tag)
Concentrazione
1 mg/mL
N° accesso UniProt
Temperatura di conservazione
−20°C
Informazioni sul gene
human immunodeficiency virus 1 ... gag(155030)
Descrizione generale
Contains amino acid residues [77-436] of HIV-1 gag antigen.
The human immunodeficiency virus 1 (HIV−1) gag antigen is a recombinant 39 kDa protein encoded by the GAG gene of the human immunodeficiency virus (HIV) type I expressed in and isolated from Escherichia coli. It has a β−galactosidase tag (114 kDa) on the N−terminus. HIV-1 Gag exists as monomers or lower-order oligomers in the cytosol. At the plasma membrane, it forms higher-order multimers.
Applicazioni
HIV-1 gag has been used in the coculture with an autologous cluster of differentiation T (CD4+ T) to study the ability of monocyte-derived dendritic cells (MDC1) to induce human immunodeficiency virus 1 (HIV−1) latency reversal (LR).
Azioni biochim/fisiol
Positive control for HIV antibodies.
The human immunodeficiency virus 1 (HIV−1) gag (p24) antigen reacts strongly with HIV positive serum and may be used as a positive control for evaluating antibodies to HIV. At transcription sites, HIV-1 Gag creates ribonucleoprotein complexes with unspliced viral RNA. The HIV-1 Gag protein is accountable for packaging genomic RNA (gRNA) and the assembly of immature viral particles.
Stato fisico
Solution in 8 M urea, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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C T Wang et al.
Journal of medical virology, 59(2), 180-188 (1999-08-25)
The incorporation of human immunodeficiency virus type 1 (HIV-1) Gag-beta-galactosidase (Gag-beta-gal; GBG) fusion proteins into HIV virus-like particles in the presence of HIV Gag proteins was studied. HIV Gag-beta-gal fusion constructs were cotransfected individually into COS7 cells with or without
Samantha Sarni et al.
The Journal of biological chemistry, 295(42), 14391-14401 (2020-08-21)
The HIV-1 Gag protein is responsible for genomic RNA (gRNA) packaging and immature viral particle assembly. Although the presence of gRNA in virions is required for viral infectivity, in its absence, Gag can assemble around cellular RNAs and form particles
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