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GERPN810

ECL™ DualVue Western Markers

Cytiva RPN810, sufficient for 25 blots

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About This Item

Codice UNSPSC:
41105331
NACRES:
NA.25

Stato

solution

impiego

sufficient for 25 blots

Caratteristiche

wetted part: no

Produttore/marchio commerciale

Cytiva RPN810

Confezionamento

pkg of 25 loadings

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

ECL™ DualVue Western Blotting Markers contain both colored proteins and tagged proteins that introduce convenience and reliability and facilitate accurate size determination of proteins on Western blots.

Applicazioni

Amersham™ ECL™ DualVue Western Blotting Markers perform two key functions. First, the prestained marker Proteins confirm Protein transfer in addition to clearly defining blot orientation. Second, the recombinant tagged Proteins are detected on film or by CCD imaging in conjunction with the target Protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target Protein(s) and markers.

Amersham™ ECL™ DualVue Western Blotting Markers contain a mixture of three prestained colored markers of defined molecular weight (from 15 000 to 100 000) and seven tagged recombinant Proteins (molecular weights from 15 000 to 150 000). Each pack contains ECL™ DualVue markers (125 μL) and S-Protein-HRP conjugate (50 μL), sufficient for 25 standard gel loadings.

Caratteristiche e vantaggi

  • Detect Proteins on gel and membrane as well as on film.
  • Prestained markers indicate efficiency of Protein transfer to membrane and blot orientation.
  • Tagged recombinant Protein markers allow accurate calculation of molecular weight of target Protein(s).
  • Product is compatible with any HRP substrate as well as both PVDF and nitrocellulose membrane types.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva

Codice della classe di stoccaggio

12 - Non Combustible Liquids

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Certificati d'analisi (COA)

Lot/Batch Number

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eLife, 9 (2020-04-22)
As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Khan et al., 2015), that described how we intended to replicate selected experiments from the paper "A coding-independent function of gene and pseudogene mRNAs regulates tumour biology"

Articoli

Troubleshooting of Detection Methods of GST-tagged Proteins

Troubleshooting of Detection Methods of GST-tagged Proteins

Protocolli

Chemiluminescence Detection with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select

This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.