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GE28-9670-56

Protein A Mag Sepharose™ Xtra

Cytiva 28-9670-56, pack of 2 × 1 mL

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

native protein A

Confezionamento

pack of 2 × 1 mL

Produttore/marchio commerciale

Cytiva 28-9670-56

Condizioni di stoccaggio

(20% Ethanol, 10% medium slurry)

Parametri

Room temperature temp. range

Matrice

paramagnetic, spherical, highly cross-linked agarose particles

Capacità

27 mg binding capacity(human IgG/ml gel)

Temperatura di conservazione

2-8°C

Descrizione generale

protein A Mag Sepharose™ are magnetic beads designed to simplify small-scale purification and screening of antibodies. Based on Sepharose™ with native protein A as ligand.

Applicazioni

Protein A Mag Sepharose™ Xtra magnetic beads are designed for rapid, small-scale purification and screening of monoclonal and polyclonal antibodies from serum and cell supernatants. The high capacity, > 27 mg human IgG/mL medium allow for the efficient capture of the antibody with high yield and purity. Approximately 350 μg purified human IgG can be obtained from each purification run.

Robust parallel screening of antibodies with high reproducibility.

Together with MagRack 6, a separation tool for handling the beads in microcentrifuge tubes, up to six samples can be processed in parallel. You can easily screen a larger number of samples in parallel with high throughput on a robotic device.

Caratteristiche e vantaggi

  • Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized high capacity for efficient small-scale purification and screening of antibodies from various species
  • High purity and yield

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Qingzhou Ji et al.
Nucleic acids research, 42(10), 6158-6167 (2014-05-06)
Artificial transcription factors are powerful tools for regulating gene expression. Here we report results with engineered zinc-finger transcription factors (ZF-TFs) targeting four protein-coding genes, OCT4, SOX2, KLF4 and c-MYC, and one noncoding ribonucleic acid (RNA) gene, the microRNA (miRNA) miR302/367

Articoli

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protocolli

Performing a Purification of IgG Antibodies with Protein A Mag Sepharose® Xtra or Protein G Mag Sepharose® Xtra

This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

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