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GE28-9031-30

Streptavidin High Performance Spintrap™

Cytiva 28-9031-30, pack of 16 columns

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.25

Caratteristiche

wetted part: no

Confezionamento

pack of 16 columns

Produttore/marchio commerciale

Cytiva 28-9031-30

Condizioni di stoccaggio

(20% Ehtanol)
 (20% Ethanol)

Volume del letto

800 μL

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning

2-10.5

working range

4-9

Capacità

>300 nmol/mL binding capacity(biotin/ml medium 6 mg biotinylated BSA/ml medium)
 binding capacity(>300 nmol biotin/ml medium 6 mg biotinylated BSA/ml medium)

Temperatura di conservazione

2-8°C

Descrizione generale

Streptavidin HP SpinTrap™ are prepacked, single-use spin columns and 96-well filter plates for the preparation of protein samples and enrichment of proteins of interest from clarified cell lysates and biological fluids.

Applicazioni

Streptavidin HP SpinTrap™ can be used for Protein enrichment, where a biotinylated antibody (or similar affinity molecule) is attached to the streptavidin and the Protein of interest is enriched through the affinity interaction with the antibody. The columns can also be used for the direct enrichment of Proteins that are biotinylated. By binding a specific biotinylated Protein to the column, Protein-Protein interactions can also be investigated.

Caratteristiche e vantaggi

  • For simple, small-scale Protein enrichment through immobilized biotinylated biomolecules.
  • Fast and flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
  • Useful for exploiting either the strong interaction of biotin and streptavidin or the somewhat weaker interaction of 2-iminobiotin and streptavidin.
  • Each column is packed with Streptavidin Sepharose™ High Performance for reproducibility and high performance.
  • Easy scale-up with HiTrap® Streptavidin HP prepacked columns.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Protocolli

Purification or Removal of Biotin and Biotinylated Substances

This page shows how to purify or remove biotin and biotinylated substances with Streptavidin Sepharose High Performance from GE Healthcare.

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules

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