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GE28-9042-67

illustra™ Plasmid Midi (25)

Cytiva 28-9042-67, pack of 1 × 25 pieces

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About This Item

Codice UNSPSC:
41105501
NACRES:
NA.52

Confezionamento

pack of 1 × 25 pieces

Produttore/marchio commerciale

Cytiva 28-9042-67

Descrizione generale

PlasmidPrep Midi Flow Kit is a gravity flow plasmid prep kit designed for the purification of large yields of transfection-grade plasmid DNA that is proportional to the input sample volume.

The mechanism of adding a DNA plasmid into a mammalian cell is known as plasmid transfection, and for it to work, transfection-grade plasmid DNA is needed. To obtain efficient gene transfer by transfection, plasmid DNA can be complexed with lipid reagents for more efficient delivery into the cell′s nucleus, essential for subsequent protein expression of the gene of interest.

PlasmidPrep Midi Flow Kit can be used to process bacterial sample volumes of 25 to 50 mL for high copy number plasmid DNA and up to 500 mL for low copy number plasmid DNA. The kit consists of a high-performance, anion exchange purification medium that combines excellent flow characteristics with an exceptional capacity and specificity for plasmid DNA.

PlasmidPrep Midi Flow Kit contains 250 fast-flow plasmid columns, resuspension, lysis, neutralization, wash, elution, and Tris-EDTA (TE) buffers, and a full protocol booklet with a detachable, quick reference protocol card.

Caratteristiche e vantaggi

  • Provides plasmid DNA with excellent purity, > 70% (strain-dependent) of supercoiled plasmid DNA
  • Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns
  • Increased ease of use due to minimal pipetting volume changes, color-coded kit components, and pre-equilibrated columns.
  • Consistently produces pure plasmid DNA with low levels of endotoxin contamination, < 10 endotoxin units (EU) per μg of plasmid DNA

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

illustra is a trademark of Cytiva

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

General Considerations for Plasmid DNA Preparation

This page shows Plasmid DNA purification using illustra plasmidPrep Kits from GE Healthcare.

Downstream Applications for Plasmid DNA and Substrates

This page shows downstream applications for plasmid DNA and substances that may interfere in each.

Plasmid DNA Preparation Troubleshooting

This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.

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