GE17-5079-01

HiTrap^® rprotein A Fast Flow

Cytiva 17-5079-01, pack of 5 × 1 mL

• Codice UNSPSC: 23151816
• NACRES: NA.56

Proprietà

• ligand: recombinant protein A (E. coli)
• Confezionamento: pack of 5 × 1 mL
• Produttore/marchio commerciale: Cytiva 17-5079-01
• Condizioni di stoccaggio: (20% Ehtanol); (20% Ethanol)
• Parametri: <4 mL/min flow rate; 42 psi
• Dimensioni del letto: 7 mm × 25 mm
• Volume del letto: 1 mL
• Diam. int. colonna: 7 mm
• Matrice: 4% cross-linked agarose
• Dimensione particelle: 60-165 μm
• Diametro medio: 90 μm (d50v)
• cleaning: 2-11
• Intervallo di esercizio: 3-10
• Capacità: ~50 mg binding capacity (human lgG/ml medium)
• Compatibilità: suitable for bioprocess medium
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Capacity of HiTrap rProtein A for some monoclonal antibodies. Running conditions: Binding buffer: 20 mM sodium phosphate (incl. 3 M NaCl for IgG), pH 7.0. Elution buffer: 0.1 M sodium citrate, pH 3.0.

HiTrap^® rProtein A FF columns are prepacked with rProtein A Sepharose^™ Fast Flow for purification and fractionation of IgG subclasses and IgG from different sources, including cell supernatants and ascites.

The recombinant protein A ligand (produced in E. coli) is coupled to Sepharose^™ Fast Flow by a technique which generates a stable thioether linkage between rProtein A and the base matrix. The coupling technique is optimized to give high binding capacity for IgG.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Caratteristiche e vantaggi

• Quick, convenient preparative purification of monoclonal antibodies.
• Well-established protein A-based medium used in many approved MAb processes.
• Recombinant protein A exhibits similar Fc region specificity to that of native protein A but shows enhanced binding capacity and fewer regulatory concerns due to the total absence of mammalian culture in the ligand production and purification.
• Simple operations with a syringe, pump, or high-performance chromatography system, such as AKTA design.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

Informazioni sulla sicurezza

• Pittogrammi: GHS02
• Avvertenze: Warning
• Indicazioni di pericolo: H226
• Consigli di prudenza: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Codice della classe di stoccaggio: 3 - Flammable liquids