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Key Documents

GE17-1235-01

Immobiline® Drystrip pH 3-10 NL, 18 cm

Cytiva 17-1235-01, pack of 12 strips

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About This Item

Codice UNSPSC:
41105323
NACRES:
NA.25

Forma fisica

solid

Caratteristiche

wetted part: no

Confezionamento

pack of 12 strips

Produttore/marchio commerciale

Cytiva 17-1235-01

Colore

Colorless

Solubilità

cold water: soluble (Insoluble in cold and hot water)

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Descrizione generale

Immobiline® DryStrip gels (IPG strips) are used for isoelectric focusing (IEF), run as the first dimension of 2-D electrophoresis or as a separate application.

Applicazioni

Immobiline® DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow-, medium-, and wide-range IPG strips available. The five narrow pH range (1 pH unit), 24-cm IPG strips deliver the highest resolution and Protein loading capacity available, which results in well separated spots with high Protein content after the second-dimension separation.

The pH 3–11 NL strip covers most Proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the Proteins evenly over the gel length to obtain maximal resolution.

Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of Protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan IPGphor 3 IEF system or Multiphor II flatbed system.

Caratteristiche e vantaggi

  • Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
  • Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
  • Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total Protein distribution.
  • For more detailed studies or when using greater Protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
  • Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
  • Use matching IPG buffers for optimized running conditions.
  • Use IPG strips directly after reswelling without further manipulation.
  • Barcoded strips for convenient sample and batch tracking.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Ettan is a trademark of Cytiva
Immobiline is a registered trademark of Cytiva

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 2


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.

Protocolli

This page describes troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from GE Healthcare.

Rehydration Solutions

This page describes two-dimensional separation of protein samples with GE Healthcare products.

Contenuto correlato

This page shows how to apply equilibrated Immobiline DryStrip gels

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