GE17-0406-01
HiTrap® Heparin High Performance
Cytiva 17-0406-01, pack of 5 × 1 mL
Sinonimo/i:
Heparin Affinity Column
About This Item
Prodotti consigliati
ligand
porcine heparin
Confezionamento
pack of 5 × 1 mL
Produttore/marchio commerciale
Cytiva 17-0406-01
Parametri
<4 mL/min flow rate
42 psi
Dimensioni del letto
7 mm × 25 mm
Volume del letto
1 mL
Diam. int. colonna
7 mm
Misura
Dimens. media delle particelle
34 μm
cleaning
5-10
working range
5-10
Categorie correlate
Descrizione generale
Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.
Heparin is coupled to the Sepharose™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.
Caratteristiche e vantaggi
- Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
- Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
- Simple operation with a syringe, a pump or a chromatography system such as AKTA design
- Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.
Stoccaggio e stabilità
Risultati analitici
Altre note
Note legali
Prodotti correlati
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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