GE17-0300-10
Ficoll® PM 400
Cytiva 17-0300-10, pack of 100 g
Sinonimo/i:
Density gradient medium
About This Item
Prodotti consigliati
Forma fisica
solid
spray-dried
Confezionamento
pack of 100 g
Produttore/marchio commerciale
Cytiva 17-0300-10
Colore
White
Solubilità
soluble
Descrizione generale
Caratteristiche e vantaggi
- For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
- Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution.
- Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
- Better osmotic properties than sucrose.
- Preserves functional and morphological integrity.
- Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
- Does not penetrate biological membranes.
- Serves as the raw material for preparation of Ficoll-Paque gradients.
- Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.
Stoccaggio e stabilità
Risultati analitici
Note legali
Codice della classe di stoccaggio
13 - Non Combustible Solids
Certificati d'analisi (COA)
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Articoli
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The table was compiled to assist the researcher in selecting references most likely to contain relevant information regarding use of Percoll in various cell types.
The effects of the mononuclear cell separation method described by Bøyum procedure are noted below, since research situations may arise in which they are significant.
Protocolli
The following procedure has been evaluated with Ficoll-Paque PLUS and is recommended for separation of normal blood samples for maximum reproducibility.
How to use percoll to prepare a gradient
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