G6046

Anti-G3BP antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinonimo/i: Anti-G3BP1, Anti-GAP SH3 domain-binding protein, Anti-HDH-VIII, Anti-Ras-GTPase-activating protein SH3-domain binding protein 1, Anti-human DNA helicase VIII
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: rabbit
• Livello qualitativo: 200
• Coniugato: unconjugated
• Forma dell’anticorpo: affinity isolated antibody
• Tipo di anticorpo: primary antibodies
• Clone: polyclonal
• Forma fisica: buffered aqueous solution
• PM: antigen ~68 kDa
• Reattività contro le specie: human, mouse (predicted), rat (predicted)
• Concentrazione: ~1.0 mg/mL
• tecniche: indirect immunofluorescence: 2-5 μg/mL using human HeLa cells; western blot: 0.1-0.2 μg/mL using whole extract of human U-2-OS cells
• N° accesso UniProt: Q13283
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... G3BP1(10146) ; mouse ... G3bp1(27041) ; rat ... G3bp1(171092)

Descrizione

Descrizione generale

G3BP is a RNA-binding protein that contains a RNA binding domain, the RRM-type domain, at the carboxyl-terminal. The N-terminal domain is homologous to nuclear transporter factor 2 (NTF2) and the central domain is rich in acidic residues. The RRM domain is involved in the binding of G3BP to specific RNA sequences. This helps G3BP to function as a CA dinucleotide-specific endoribonuclease. G3BP′s subcellular localization and protein-protein interactions are mediated by the phosphorylation of G3BP at Ser¹⁴⁹, near NTF2-like domain. G3BP is found in abundance in many kinds of malignant tumors such as lung, colon, gastric, and breast cancer.

Applicazioni

Anti-G3BP antibody produced in rabbit has been used in co-immunoprecipitation assay.

Anti-G3BP antibody produced in rabbit has been used in

• immunofluorescence analysis
• immunostaining
• immunoblotting
• immunoprecipitation

Azioni biochim/fisiol

G3BP (Ras-GTPase-activating protein SH3 domain binding protein 1) is a endoribonuclease that is phosphorylation-dependent and specific to single strand. It cleaves between cytosine and adenine (CA). It binds to the SH3 domain of RasGAP. It forms a link between RasGAP-mediated signaling pathway and RNA turnover. G3BP is required for the assembly of stress granules (SGs), which are involved in the regulation of mRNA metabolism during stress. It may be used as a cytoplasmic SG marker.

Ras-GAP SH3 domain binding protein (G3BP) is overexpressed in many kinds of malignant tumors such as breast, colon, lung and gastric breast cancer. G3BP expression is correlated with lymph node metastasis in breast cancer.

Descrizione del bersaglio

G3BP encodes one of the DNA-unwinding enzymes which prefers partially unwound 3′-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Nota sulla preparazione

In immunoblotting assays, it is highly recommended to dilute the antibody in 5% NFDM (w/v).

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)