F0552

Fas Ligand from mouse

>95% (SDS-PAGE), recombinant, expressed in mouse NSO cells, lyophilized powder

• Numero MDL: MFCD00285792
• Codice UNSPSC: 12352200
• NACRES: NA.32

Proprietà

• Ricombinante: expressed in mouse NSO cells
• Livello qualitativo: 100
• Saggio: >95% (SDS-PAGE)
• Forma fisica: lyophilized powder
• PM: monomer calculated mol wt ~18 kDa; 28-32 kDa by SDS-PAGE
• Impurezze: endotoxin, tested
• N° accesso UniProt: P41047
• Temperatura di conservazione: −20°C
• Informazioni sul gene: mouse ... Fasl(14103)

Descrizione

Descrizione generale

FASLG (Fas ligand) acts as a ligand for Fas receptor, and is a major protein involved in programmed cell death, apoptosis. Soluble Fas (sFAS) is usually detected in plasma prior to apoptosis.

Applicazioni

Fas Ligand (FASLG) from mouse has been used for-

• the induction of apoptosis in PC12 cells and
• the induction of migration in BV-2 murine microglial cells.

Azioni biochim/fisiol

FASLG (Fas ligand) and Fas receptor constitute the basic elements in apoptosis. Interaction of FASLG with Fas receptor leads to activation of caspase-8. This caspase in turn leads to activation of effector caspases such as caspase-3, -6 and -7. This cascade results in the hydrolysis of nuclear and cytoplasmic components. Expression of FASLG is induced by nuclear factor-κB (NFκB). NFκB/FASLG pathway facilitates the suppression of p,p′-DDT (dichlorodiphenoxytrichloroethane)-induced cell toxicity by vitamin C and E. In CD4⁺ T cells, this protein is expressed on stimulus by T-cell receptor (TCR), both during normal and pathological conditions, such as alcohol exposure.

Fas ligand, a protein belonging to the tumor necrosis factor (TNF) family of cytokines, induces apoptosis in cells expressing the cell membrane receptor Fas (CD95/Apo-1).

Altre note

Mouse Fas Ligand, N-terminal 6X histidine-tagged, encodes amino acid residues 132-279.

Stato fisico

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Risultati analitici

Measured by its ability to induce apoptosis in Jurkat cells.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable