ATDC5

99072806

• Sinonimo/i: ATDC-5 Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse unknown/unspecified
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse 129 teratocarcinoma AT805 derived

Descrizione della linea cellulare

The chondrogenic cell line ATDC5 has been derived from teratocarcinoma AT805. Cells showed a sequential transition of phenotype in vitro. They encompass stages from mesenchymal condensation to calcification. Bone morphogenetic protein-2 (BMP-2) stimulated the sequential progression of early- and late-phase differentiation. Activation of parathyroid hormone (PTH)/PTH-related peptide (PTHrP) receptor resulted in inhibition of this differentiation capacity.

Applicazioni

ATDC5 has been used to study cartilage differentiation.

Terreno di coltura

DMEM: Ham′s F12 (1:1) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) i.e. seeding at 1-3 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA;CO₂; 37°C subculture every 2-3 days.

Altre note

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