NCI-H69/LX4

96042329, human lung, Not specified

• Codice UNSPSC: 41106514

Proprietà

• product name: NCI-H69/LX4, 96042329
• Origine biologica: human lung
• Modalità di accrescimento: Aggregates in suspension
• Cariotipo: Not specified
• Morfologia: Not specified
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Caucasian small cell lung carcinoma, drug-resistant

Descrizione della linea cellulare

NCI-H69/LX4 has been established by exposure of the parent line, H69 (Sigma Catalogue number. 91091802) to doxorubicin, also known as adriamycin. The line hyperexpresses P-glycoprotein and demonstrates a multidrug resistant drug accumulation deficit. It is recommended to culture the cells without drug after resuscitation until the first passage.

Applicazioni

Analysis of acquired drug-resistance, study of small cell lung cancer

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Terreno di coltura

RPMI + 2mM Glutamine + 0.4μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) by repeated pipetting of aggregates or scraping; 5% CO₂; 37°C

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.