RCE

95081046, rabbit eye, Epithelial-like

• Codice UNSPSC: 41106514

Proprietà

• product name: RCE, 95081046
• Origine biologica: rabbit eye
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial-like
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rabbit corneal epithelial cells, SV40 transformed

Descrizione della linea cellulare

The RCE cell line, also known as K′RCE, was established after infection of primary cultured rabbit (New Zealand albino) corneal epithelial cells with a recombinant SV40 vector. The cells exhibit a cobblestone-like appearance and stratification, including desmosome formation and microvilli development. Expression of keratin AE1 was always detectable whereas AE5 was expressed only at confluency.

Applicazioni

Corneal epithelial research

Terreno di coltura

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 5μg/ml Insulin + 10ng/ml EGF + 0.1μg/ml Cholera toxin + 0.5% Dimethyl Sulphoxide (DMSO) + 15% Foetal Bovine Serum (FBS) (Heat Inactivated).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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