AFRC 58D6

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: Horse x Mouse/Horse Hybridoma
• Confezionamento: tube of 5 μg 94120827-DNA-5UG; pkg of vial of cells 94120827-1VL
• ImmunoDon: Horse sub-mandibular lymph node
• Mieloma: AFRC 17D1
• Reattività: Anti-Haemagglutinin, influenza
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Horse sub-mandibular lymph node x AFRC 17D1

Descrizione della linea cellulare

The heterohybridoma AFRC 58D6 is one of 8 hybridoma cell lines obtained using lymphocytes from ponies immunised with influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)). For the establishment of AFRC 58D6 horse cells from the sub-mandibular lymph node have been fused with the non-Ig-secreting heterohybridoma AFRC 17D1 (Sigma Catalogue number. 94120825).

Immunogeno

Influenza A equine 2 virus

Applicazioni

Haemagglutinin detection

The isotype of the monoclonal antibody produced by this cell line is horse IgG.

Terreno di coltura

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

Altre note

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