HF1-5

94101909, rat liver, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: HF1-5, 94101909
• Origine biologica: rat liver
• Modalità di accrescimento: Adherent
• Cariotipo: 100 chromosomes
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat hepatoma Hybrid [H5xFao]

Descrizione della linea cellulare

HF1-5 is a subclone of HF1 selected for it′s ability to grow in glucose free medium. The entire group of hepatic functions are re-expressed. NB. The cells are difficult to cultivate, they do not like either low or high density and should be transferred frequently even though they grow slowly.

Applicazioni

Gene analysis and liver function expression.

Terreno di coltura

Coon′s modified Ham′s F12 (or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split semi-confluent (60%-70%) cultures 1:5 i.e. seeding at 3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 37°C. Saturation density is reached at 5x1000,000 cells/10cm dish.

Altre note

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