ND7/23 Cell Line

92090903, Mouse x Rat Hybridoma nerve, Neuronal

• Sinonimo/i: ND7/23 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: ND7/23 Cell Line, 92090903, Mouse neuroblastoma X Rat neuron hybrid
• Origine biologica: Mouse x Rat Hybridoma nerve
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Neuronal
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse neuroblastoma x Rat neurone hybrid

Descrizione della linea cellulare

Mouse neuroblastoma (N18 tg 2) x rat dorsal root ganglion neurone hybrid cell line produced by PEG mediated cell fusion.

Applicazioni

Mouse neuroblastoma (N18 tg 2) and rat dorsal root glanglia produced by PEG-mediated fusion.

Recommended culture medium: DMEM + 2 mM L-Glutamine + 10% FBS

Subculture: Split at 70-80% confluency, approx. 1:3 to 1:6, seed at 1-3x10⁴ viable cells/cm² at 37°C and 5% CO₂. Dissociate cells by aspiration or gentle tapping. Cells are lightly adherent, no trypsinization is necessary.

Terreno di coltura

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² by either aspirating or gently tapping the cells loose; 5% CO₂; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.