NCTC 4093

90102530, mouse embryo, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: NCTC 4093, 90102530
• Origine biologica: mouse embryo
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 71-73; hypotetraploid
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C57B1/KaLw embryo

Descrizione della linea cellulare

Derived by cloning from a parent line produced from normal mouse embryo tissue (C57B1/KaLw). The cells have spontaneously transformed in vitro. The cells are negative for polyoma virus infection but are susceptible to the virus.

Applicazioni

Transformation and virus studies: Polyoma virus

Terreno di coltura

NCTC 135 100% + 2mM Glutamine.

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using mechanical methods to dislodge the cells (e.g. scraping); 5% CO₂; 37°C.

Altre note

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