NCTC clone 1469

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 88111403, mouse liver, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: mouse liver
• Confezionamento: tube of 5 μg 88111403-DNA-5UG; pkg of vial of cells 88111403-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse C3H/An liver

Descrizione della linea cellulare

Derived from mouse liver cell line NCTC 721 which was obtained from normal liver of a 2 day old C3H/An mouse. The cells have been used in biochemical and nutritional studies. The cells are susceptible to VSV (Indiana strain).

Applicazioni

Cell suspension systems. Biochemical, nutritional and virus studies. VSV(Indiana)

Terreno di coltura

NCTC 135 + 2mM Glutamine + 10% Horse Serum (HS)

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using only mechanical techniques, such as scraper, do not use trypsin, 5-10CO₂; 37°C. Change media every 3 days. Freeze in 10% glycerol/90% FBS. Cells are shed into the medium giving it a turbid appearan

Altre note

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