LoVo Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87060101, human colon (supraclavicular lymph node metastasis), Epithelial

• Sinonimo/i: LoVo Cells
• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: human colon (supraclavicular lymph node metastasis)
• Confezionamento: tube of 5 μg 87060101-DNA-5UG; pkg of vial of cells 87060101-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Modal no. 49, (2n=46)
• Morfologia: Epithelial
• Prodotti: Carcinoembryonic antigen (CEA)
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: metastasis
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human colon adenocarcinoma

Descrizione della linea cellulare

Derived from a metastatic tumour in the left supraclavicular region of a 56 year old male with adenocarcinoma of the colon. The cells produce carcino embryonic antigen (CEA).

Applicazioni

Human LoVo cell line is part of the three-dimensional (3D) heterotypic spheroid model developed to assess cancer immunotherapy agents in vitro. It has been used to test the in vitro cytotoxic property and anti-proliferative property of C. lechleri stem bark essential oil.

Tumourigenicity studies

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13,14
D13S317: 8,11
D16S539: 9,12
D5S818: 11,13
D7S820: 10,11
THO1: 9.3
TPOX: 8,9
vWA: 17,18

Terreno di coltura

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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