H6/31HL

85102214, Reactivity: Anti-IgD, mouse

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Mieloma: P3X63Ag8
• Reattività: Anti-IgD, mouse
• tecniche: cell culture | mammalian: suitable
• Isotipo: IgM
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Mouse P3X63Ag8 x Mouse BALB/c spleen

Descrizione della linea cellulare

The antibody is directed against ′IgD-like′ molecules of mice with IgB haplotype. The target antigen has a molecular weight of 185kDa and is found on a high proportion of mature lymphoid cells, particularly on cells from Peyer′s patches. It is not detected on thymus cells.

Immunogeno

Two grafts of full thickness skin from B10 mice followed by B10 spleen cells

Terreno di coltura

DMEM + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS)

Mantenimento delle subcolture

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C.

Altre note

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Esclusione di responsabilità

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.