HTC (BUdR)

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Codice UNSPSC: 41106514

Proprietà

• Origine biologica: rat liver
• Confezionamento: tube of 5 μg 85061110-DNA-5UG; pkg of vial of cells 85061110-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: 2n = 66
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Rat liver hepatoma

Descrizione della linea cellulare

Clone of the original HTC hepatoma (Sigma Catalogue number. 93120108) rendered resistant to 10ug/ml BUdR, which must be grown in HAT. The cells are used in enzyme induction studies.

Applicazioni

Enzymatic studies

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1%HAT + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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