HeLa B

85060701, human cervix, Epithelial

• Codice UNSPSC: 41106514

Proprietà

• product name: HeLa B, 85060701
• Origine biologica: human cervix
• Modalità di accrescimento: Adherent
• Cariotipo: Aneuploid
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human cervix carcinoma

Descrizione della linea cellulare

A derivative of HeLa, the first aneuploid, epithelial-like line derived from human tissue and maintained by serial subculture. Identified as a contaminant in many subsequently derived lines, due to poor culture techniques. Susceptible to poliovirus 1 and Ad 3.

Applicazioni

Tumourigenicity and virus studies: poliovirus 1 and Ad 3

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Altre note

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