DAUDI Cell Line human

85011437, human blood, Lymphoblast

• Sinonimo/i: DAUDI Cell Culture, Human DAUDI Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: DAUDI Cell Line human, 85011437, African-American Burkitt′s lymphoma, from human blood
• Origine biologica: human blood
• Modalità di accrescimento: Suspension
• Cariotipo: 2n = 46, diploid
• Morfologia: Lymphoblast
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Human Burkitt′s lymphoma

Descrizione della linea cellulare

Derived from a 16 year old male. Positive for EBNA, carries EBV markers, complement receptors, surface bound immunoglobulin and surface markers for the Fc fragment of IgG. The cells should be handled under laboratory containment level 2. Ethnicity: Black.

Applicazioni

Tumourigenicity and Virus studies: EBV used to study leukemogenesis mechanisms.

Profilo DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 11,12
D16S539: 10,12
D5S818: 8,13
D7S820: 8,10
THO1: 6,7
TPOX: 8,11
vWA: 15,17

Terreno di coltura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Maintain cultures between 2-9x100,000 cells/ml; 5% CO₂; 37°C. Will increase 12 fold within 10-14 days provided fresh medium is added at 48-72 hour intervals. Cell growth is slow on recovery from frozen. Resuscitate using 20% FBS and keep culture flask standing up until exponential growth is established.

Altre note

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