LIM1899 cell line human

12062001, human colon, Epithelial; cells are small rounded and grow in clusters

• Sinonimo/i: LIM 1899, LIM-1899
• Codice UNSPSC: 41106514

Proprietà

• product name: LIM1899 cell line human, 12062001
• Origine biologica: human colon
• Modalità di accrescimento: adherent
• Morfologia: Epithelial; cells are small rounded and grow in clusters
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Descrizione della linea cellulare

Columnar cell adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0163.

Terreno di coltura

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM

Mantenimento delle subcolture

Optimal split ratio 1:8 (seeding density 1 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM1899 requires the growth medium to be changed 3 times each week.

Altre note

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