HRA-19a1.1

9071517, human colon, Epithelial

• Sinonimo/i: HRA, HRA19
• Codice UNSPSC: 41106514

Proprietà

• Nome del prodotto: HRA-19a1.1, 09071517
• Origine biologica: human colon
• Confezionamento: tube of 5 μg 09071517-DNA-5UG; pkg of vial of cells 09071517-1VL
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Not specified
• tecniche: cell culture | mammalian: suitable
• Malattie correlate: cancer
• Condizioni di spedizione: dry ice

Descrizione

Origine della linea cellulare

Human colorectal adenocarcinoma

Descrizione della linea cellulare

The HRA-19 cell line was established from a primary human rectal adenocarcinoma present in a 66-year old male. The adenocarcinoma was well differentiated and determined to be Dukes′ stage B. The HRA-19a1.1 cell line is a clone of HRA-19. HRA-19 has stem cell properties and undergoes a multilineage differentiation in serum free medium.

Profilo DNA

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,12
D16S539: 11
D5S818: 11
D7S820: 9,12
THO1: 8
TPOX: 11
vWA: 16

Terreno di coltura

DMEM - high glucose (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:5 to 1:6 weekly using 0.05% trypsin/EDTA; 7.5% CO₂; 37 °C. Suggested seeding density 2-4x10,000 cells/cm².

Altre note

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Esclusione di responsabilità

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.