MDCK-SIAT1 Cell Line canine

5071502, canine kidney, Epithelial

• Sinonimo/i: MDCK-SIAT1 Cells
• Codice UNSPSC: 41106514

Proprietà

• product name: MDCK-SIAT1 Cell Line canine, 05071502
• Origine biologica: canine kidney
• Descrizione: Canine Cocker Spaniel Kidney Sialic Acid Over Expression
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Epithelial
• Prodotti: Not specified
• Recettori: Enhanced expression of 6-linked sialic acids
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Canine Cocker Spaniel Kidney Sialic Acid Over Expression

Descrizione della linea cellulare

This cell line has been derived by the stable transfection of MDCK cells with the cDNA of human 2,6-sialtransferase (SIAT1). The cells express two fold?higher amounts of 6-linked sialic acids and two fold-lower amounts of 3-linked sialic acids than parent MDCK cells. MDCK-SIAT1 cells are presented as a suitable system for testing the sensitivity of human influenza virus to neuraminidase inhibitors (NAI) due to their over expression of sialyl-α2,6-galactose moieties.

Terreno di coltura

DMEM + 2mM Glutamine + 1mg/ml G418 + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (85-95 %) 1:10 to 1:20 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 37°C; 5% CO₂.

Altre note

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