IIC9 (CH0)

30814, hamster embryo, Fibroblast

• Codice UNSPSC: 41106514

Proprietà

• product name: IIC9 (CH0), 00030814
• Origine biologica: hamster embryo
• Modalità di accrescimento: Adherent
• Cariotipo: Not specified
• Morfologia: Fibroblast
• Prodotti: Not specified
• Recettori: PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
• tecniche: cell culture | mammalian: suitable
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −196°C

Descrizione

Origine della linea cellulare

Chinese hamster embryo fibroblast

Descrizione della linea cellulare

The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.

Applicazioni

IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.

Terreno di coltura

DMEM:F12 (1:1) (D8062) + 5% FBS / FCS (F2442) + 2mM L-Glutamine (G7513).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin /EDTA; 37°C; 5% CO₂.

Altre note

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