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CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

Sinonimo/i:

Caspase-3 Activity Fluorometric Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali


About This Item

Numero CE:
Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

Livello qualitativo

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CASP3(836)

Descrizione generale

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Applicazioni

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Definizione di unità

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Solo come componenti del kit

N° Catalogo
Descrizione

  • Water (17-18 megaOhm) 125 mL

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS

  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS

  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS

  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

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Avvertenze

Warning

Indicazioni di pericolo

Classi di pericolo

Eye Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3


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