C1805

Anti-CD4 antibody, Mouse monoclonal

clone Q4120, purified from hybridoma cell culture

• Sinonimo/i: Anti-CD4 antibody, Mouse monoclonal
• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Coniugato: unconjugated
• Forma dell’anticorpo: purified from hybridoma cell culture
• Tipo di anticorpo: primary antibodies
• Clone: Q4120, monoclonal
• Stato: buffered aqueous solution
• PM: 59 kDa
• Reattività contro le specie: human
• tecniche: flow cytometry: 5 μL using 1 × 10⁶ cells
• Isotipo: IgG1
• N° accesso UniProt: P01730
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... CD4(920)

Descrizione

Descrizione generale

Monoclonal Anti-Human CD4 (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cell line NS-1 and splenocytes from Balb/c mice immunized with CD4-Transfected mouse T-cell hybridoma, 3DT, followed by CD4⁺ human T-cell CEM cells. Cluster of differentiation 4 (CD4) human cell surface glycoprotein (59kDa) belongs to the immunoglobulin superfamily. It is expressed on the helper/inducer T cell subset, which is found on the majority of peripheral blood neutrophils, most cortical and mature medullary thymocytes, microglial cells, dendritic cells and on some malignancies of T cell origin.

Specificità

Recognizes the CD4. The epitope recognized by the Q4120 clone is located on 1-183 aa and is sensitive to formalin fixation and paraffin embedding. 5th Workshop: code no. CD04.11

Immunogeno

CD4-transfected mouse T-cell hybridoma, 3DT, followed by CD4+ human T-cell CEM cells.

Applicazioni

Monoclonal Anti-CD4 antibody produced in mouse has been used in indirect immunofluorescent staining.

Azioni biochim/fisiol

Cluster of differentiation 4 (CD4) molecule binds to the major histocompatibility complex (MHC) class II molecules during the interaction of CD4⁺ T cells with antigen presenting cells or with target cells. It also serves as a high affinity cellular receptor for the GP 120 envelope glycoprotein of the human immunodeficiency virus (HIV-1, HIV-2). The CD4 molecule is involved in the adhesion of T-lymphocytes to target cells, thymic development, transmission of intracellular signals during T cell activation, and binding to polyclonal immunoglobulins. Lower levels of CD4 have been detected in monocytes, macrophages and granulocytes.

Descrizione del bersaglio

CD4 is a single chain transmembraneous glycoprotein from the immunoglobulin superfamily. It is expressed on the helper/inducer T subset, on most medullary thymocytes, on microglial, dendritic and on some malignancies of T cell origin. The antigen binds to MHC class II molecules and is associated with p56^lck protein tyrosine kinase.

Stato fisico

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable