B2787
Biotinylated Molecular Weight Marker
mol wt 6,500-180,000 Da
Sinonimo/i:
protein markers, protein molecular weight markers, protein standards
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About This Item
Codice UNSPSC:
12352200
eCl@ss:
34058011
NACRES:
NA.25
Prodotti consigliati
Stato
lyophilized powder
Livello qualitativo
impiego
vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)
PM
6,500-180,000 Da
Confezionamento
vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)
Temperatura di conservazione
−20°C
Applicazioni
For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.
Componenti
This product contains:
- α2-macroglobulin (equine serum)
- β-galactosidase (E. coli)
- phosphorylase b (rabbit muscle)
- catalase (bovine liver)
- alcohol dehydrogenase (yeast)
- carbonic anhydrase (bovine erythrocyte)
- trypsin inhibitor (soy bean)
- lysozyme (chicken egg)
- aprotinin (bovine lung)
Altre note
Contains nine biotinylated proteins (approx 0.1 mg total).
Ricostituzione
Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I clienti hanno visto anche
D Della-Penna et al.
Analytical biochemistry, 152(2), 329-332 (1986-02-01)
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)
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