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92846

Sigma-Aldrich

5-Carboxyfluorescein N-succinimidyl ester

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Formula empirica (notazione di Hill):
C25H15NO9
Numero CAS:
92557-80-7
Peso molecolare:
473.39
Beilstein:
7865828
Numero MDL:
MFCD00077322
Codice UNSPSC:
12352108
ID PubChem:
57653496
NACRES:
NA.32

Nome Commerciale

BioReagent

Livello qualitativo

100

Saggio

≥90% (HPLC)

Forma fisica

powder

Fluorescenza

λex 492 nm; λem 520 nm in 0.1 M Tris pH 8.0

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Stringa SMILE

OC(=O)c1cc(ccc1C2=C3C=CC(=O)C=C3Oc4cc(O)ccc24)C(=O)ON5C(=O)CCC5=O

InChI

1S/C25H15NO9/c27-13-2-5-16-19(10-13)34-20-11-14(28)3-6-17(20)23(16)15-4-1-12(9-18(15)24(31)32)25(33)35-26-21(29)7-8-22(26)30/h1-6,9-11,27H,7-8H2,(H,31,32)
TWOKNYRPKCLMAR-UHFFFAOYSA-N

Confezionamento

Bottomless glass bottle. Contents are inside inserted fused cone.

Altre note

Derivative of fluorescein giving stable derivative upon conjugation with biopolymers. Fluorescent probe for nucleotides; Reagent for fluorescent derivatization in capillary electrophoresis

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Bamaprasad Dutta et al.
Scientific reports, 7(1), 5765-5765 (2017-07-20)
Atherosclerosis arises from leukocyte infiltration and thickening of the artery walls and constitutes a major component of vascular disease pathology, but the molecular events underpinning this process are not fully understood. Proteins containing an Asn-Gly-Arg (NGR) motif readily undergo deamidation
Lau, S.K., et al.
Journal of Chromatography A, 809, 203-203 (1998)
G L Igloi
Analytical biochemistry, 233(1), 124-129 (1996-01-01)
A modified ribodinucleotide (named pSEEp) has been synthesized using commercially available components on a DNA synthesizer. The presence of a 3'-terminal primary amino group permits its coupling to a range of nonradioactive labels, exemplified here by fluorescein. The product of
L G Lee et al.
Nucleic acids research, 20(10), 2471-2483 (1992-05-25)
The incorporation of fluorescently labeled dideoxynucleotides by T7 DNA polymerase is optimized by the use of Mn2+, fluorescein analogs and four 2'-deoxyribonucleoside 5'-O-(1-thiotriphosphates) (dNTP alpha S's). The one-tube extension protocol was tested on single-stranded templates, as well as PCR fragments
Brandon L Blakely et al.
Nature methods, 11(12), 1229-1232 (2014-10-13)
We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to

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