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Documenti fondamentali

85051004

INT 407 (HeLa derivative) cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, human cervix, Epithelial

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About This Item

Codice UNSPSC:
41106514

Origine biologica

human cervix

Stato

liquid

Confezionamento

tube of 5 μg 85051004-DNA-5UG
pkg of vial of cells 85051004-1VL

Modalità di accrescimento

Adherent

Cariotipo

2n = 46, HeLa markers

Morfologia

Epithelial

Prodotti

Not specified

Recettori

Not specified

tecniche

cell culture | mammalian: suitable

Malattie correlate

cancer

Origine della linea cellulare

Human cervix carcinoma

Descrizione della linea cellulare

Originally derived from the jejunum and ileum of a 2 month old Caucasian embryo. The cells have a similar virus susceptibility as HeLa but show less sensitivity. They carry HeLa marker chromosomes This cell line has been found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cells must be considered as derived from HeLa. Ethnicity: Black.

Applicazioni

Virus studies

Confezionamento

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Terreno di coltura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Mantenimento delle subcolture

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm2using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections, supplied by us, are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Esclusione di responsabilità

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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The establishment of strains of human cells in tissue culture.
G HENLE et al.
Journal of immunology (Baltimore, Md. : 1950), 79(1), 54-59 (1957-07-01)

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