62309
Lipase from Pseudomonas cepacia
powder, light beige, ≥30 U/mg
Sinonimo/i:
PCL, PS Lipase, Triacylglycerol acylhydrolase, Triacylglycerol lipase
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Origine biologica
bacterial (Pseudomonas cepacia)
Livello qualitativo
Stato
powder
Attività specifica
≥30 U/mg
Condizioni di stoccaggio
(Tightly closed. Dry)
tecniche
cell based assay: suitable
Colore
light beige
Solubilità
H2O: 2 mg/mL, hazy, faintly yellow
N° accesso UniProt
Temperatura di conservazione
2-8°C
InChI
1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
QWZUIMCIEOCSJF-CHHCPSLASA-N
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Descrizione generale
Research area: cell-signaling
Lipase is a hydrolytic enzyme, found ubiquitously in nature. It belongs to the α/β-hydrolases fold family. Lipase structure contains amphipathic helical lid domain in the active site that helps in interfacial activation of protein.
Lipase is a hydrolytic enzyme, found ubiquitously in nature. It belongs to the α/β-hydrolases fold family. Lipase structure contains amphipathic helical lid domain in the active site that helps in interfacial activation of protein.
Applicazioni
Lipases are used industrially for the resolution of chiral compounds and the transesterification production of biodiesel.
Lipase from Pseudomonas cepacian has been used to:
Lipase from Pseudomonas cepacian has been used to:
- catalyze the degradation of polycaprolactone scaffold
- catalyze the hydrolysis of Morita-Baylis-Hillman acetates during enzymatic kinetic resolution of racemic Morita-Baylis-Hillman adducts
- as a standard for the generation of a calibration curve to determine the activity of lipase produced by microorganisms isolated from sludge derived from an urban wastewater treatment plant for ethanol production.
Azioni biochim/fisiol
Lipases catalyze the hydrolysis of carboxylic ester bonds in triacylglycerols to yield glycerol and free fatty acids. Tri-, di-, and monoglycerides are hydrolyzed (in decreasing order of rate). Triacylglycerol lipases specifically hydrolyze the outer links of triacylglycerols and operate exclusively on the water-lipid interface. Lipolytic products and intermediates formed during lipolysis are involved in various cell-signaling processes. Lipases have broad substrate specificity and high enantioselectivity. This property of lipase makes it a good catalyst in organic synthesis. Lipases play a vital role in fat digestion and metabolism.
Definizione di unità
1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)
Altre note
Note: When triacetin is used as substrate, the pH is 7.4. Incubation time: 60 minutes.
Chemoenzymatic synthesis of (-)-carbocyclic 7-deazaoxetanocin G
Enzima
N° Catalogo
Descrizione
Determinazione del prezzo
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Resp. Sens. 1
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Patricia Godoy et al.
Frontiers in microbiology, 9, 2634-2634 (2018-11-18)
A collection of lipase-producing microorganisms was isolated from sludge derived from an urban wastewater treatment plant. The microorganisms with the highest levels of lipase activity were selected in order to use triglycerides present in the sludge effectively and were then
Cammy K-M Chen et al.
Journal of molecular biology, 390(4), 672-685 (2009-05-19)
Several crystal structures of AFL, a novel lipase from the archaeon Archaeoglobus fulgidus, complexed with various ligands, have been determined at about 1.8 A resolution. This enzyme has optimal activity in the temperature range of 70-90 degrees C and pH
X. Chen et al.
Tetrahedron Letters, 33, 2249-2249 (1992)
Rudolf Zechner et al.
Cell metabolism, 15(3), 279-291 (2012-03-13)
Lipolysis is defined as the catabolism of triacylglycerols stored in cellular lipid droplets. Recent discoveries of essential lipolytic enzymes and characterization of numerous regulatory proteins and mechanisms have fundamentally changed our perception of lipolysis and its impact on cellular metabolism.
Enzymatic kinetic resolution of Morita-Baylis-Hillman acetates
Juma WP, et al., et al.
Tetrahedron Asymmetry, 28(9), 155-155 (2017)
Articoli
SHVO’S CATALYST: EFFICIENT EPIMERIZATION CATALYST FOR ENZYME MEDIATED DYNAMIC KINETIC RESOLUTION (DKR).
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.