308M-1

Oct-2 (MRQ-2) Mouse Monoclonal Antibody

• Codice UNSPSC: 12352203
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100; 500
• Coniugato: unconjugated
• Forma dell’anticorpo: diluted ascites fluid
• Tipo di anticorpo: primary antibodies
• Clone: MRQ-2, monoclonal
• Descrizione: For In Vitro Diagnostic Use in Select Regions (See Chart)
• Forma fisica: buffered aqueous solution
• Reattività contro le specie: human
• Confezionamento: vial of 0.1 mL concentrate (308M-14); vial of 0.5 mL concentrate (308M-15); bottle of 1.0 mL predilute (308M-17); vial of 1.0 mL concentrate (308M-16); bottle of 7.0 mL predilute (308M-18)
• Produttore/marchio commerciale: Cell Marque^™
• tecniche: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:25-1:100
• Isotipo: IgG1κ
• Controllo: tonsil
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C
• Visualizzazione: nuclear
• Informazioni sul gene: human ... POU2F2(5452)

Descrizione

Descrizione generale

Octamer-binding transcription factor-2 (Oct-2) is a transcription factor of the POU homeo-domain family. It binds to Ig gene octamer sites and plays a crucial role in B-cell development and differentiation, as well as in B-cell identity. Oct-2 is expressed in a subset of normal B-lymphocytes and is expressed in various B-cell lymphomas as well as nodular lymphocyte predominant Hodgkin lymphoma, while it is typically not expressed in the Reed–Sternberg and Hodgkin (RS/H) cells of classical Hodgkin lymphoma.


Oct-2 is a transcription factor of the POU homeo-domain family that binds to the Ig gene octamer sites, regulating B-cell-specific genes. These are involved in proliferation and differentiation and despite the scarce evidence for Oct-2 expression in T cells, it has been shown that this factor participates in transcriptional regulation during T-cell activation. Oct-2 activity is dependent on phosphorylation and alternatitive splicing, although it seems that the level of its expression can be used as a marker of B-cell lineage and differentiation. The following show high levels of Oct-2 expression: germinal center B-cells, mantle B-cells, monocytoid B-cells, and plasma cells. Various lymphomas are also positive for this marker including the following: B-chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, classic Hodgkin lymphoma.

Several studies of Oct-2 expression have shown a low level expression in pre-B, T-cell, myelomonocytic, and epithelial cell lines, whereas all mature B-cell lines display high levels of expression. Analysis of Oct-2 expression in primary Hodgkin’s lymphoma (HLs) and cell lines derived from it showed that the tumor cells analyzed had high levels of expression and activity, suggesting a common B-cell origin for all types of HLs.

Associated products: bcl-6, CD20, CD79a, PAX-5, BOB.1

Qualità

IVD

IVD

IVD

RUO

Linkage

Oct-2 Positive Control Slides, Product No. 308S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Stato fisico

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Nota sulla preparazione

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Altre note

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Note legali

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable