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Documenti fondamentali

257R-1

Sigma-Aldrich

GCDFP-15 (EP1582Y) Rabbit Monoclonal Primary Antibody

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.41

Origine biologica

rabbit

Livello qualitativo

100
500

Coniugato

unconjugated

Forma dell’anticorpo

culture supernatant

Tipo di anticorpo

primary antibodies

Clone

EP1582Y, monoclonal

Descrizione

For In Vitro Diagnostic Use in Select Regions (See Chart)

Stato

buffered aqueous solution

Reattività contro le specie

human

Confezionamento

vial of 0.1 mL concentrate (257R-14)
vial of 0.5 mL concentrate (257R-15)
bottle of 1.0 mL predilute (257R-17)
vial of 1.0 mL concentrate (257R-16)
bottle of 7.0 mL predilute (257R-18)

Produttore/marchio commerciale

Cell Marque®

tecniche

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100-1:500

Isotipo

IgG

Controllo

breast, breast carcinoma

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

Visualizzazione

cytoplasmic

Informazioni sul gene

human ... PIP(5304)

Descrizione generale

Gross cystic disease fluid protein-15 (GCDFP-15) is one of four major component proteins found in the cystic fluid obtained from patients with fibrocystic changes of the breast. GCDFP-15 is a marker of apocrine glandular differentiation in both benign and malignant mammary epithelium. This protein has a widespread distribution in apocrine glands elsewhere in the axillary and perianal tissues, as well as in the sublingual and submaxillary salivary glands. The GCDFP-15 protein is a 15 kDa glycoprotein shown to be prolactin-inducible, and the GCDFP-15 gene has been cloned. Ultrastructurally, the GCDFP-15 protein has been localized in Golgi vesicle and cytoplasmic granules. The protein is released by exocytosis at the apices of the mammary epithelial cells. The primary application for this antibody product is the identification of breast carcinoma in the metastatic setting, or fluid analysis. In a study by Wick et al. on paraffin sections of 105 breast cancers and 585 non-mammary malignancies in order to assess its value in this context, anti-GCDFP-15 was compared with another putative mammary epithelial marker, alpha-lactalbumin (ALA), with respect to sensitivity and specificity for a diagnosis of breast carcinoma. Overall, the rates of specificity and sensitivity and positive predictive value for GCDFP-15 were 95%, 74%, and 74%, respectively. A consistent congruency between the reactivity patterns of primary and metastatic breast cancers was noted for GCDFP-15. Besides mammary carcinomas, the major tumor types that express GCDFP-15 were carcinomas of the salivary glands and sweat glands. Since the latter two types of lesions are unlikely to be diagnosed as metastatic breast cancer, statistical indices were recalculated after exclusion of these three tumor types. Following this exclusion, the adjusted rate of specificity of GCDFP-15 and positive predictive value for a diagnosis of metastatic carcinoma of the breast were each 99%. Another study by Bhargava et al. has shown GCDFP-15 staining of a higher percentage of cancer cells in lobular carcinoma as compared to ductal carcinoma of the breast.

Linkage

GCDFP-15 Positive Control Slides, Product No. 257S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Stato fisico

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Nota sulla preparazione

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Altre note

For Technical Service please contact: 800-665-7284 or email: service@cellmarque.com

Note legali

Cell Marque is a registered trademark of Merck KGaA, Darmstadt, Germany

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Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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S Ansai et al.
The American Journal of dermatopathology, 17(3), 249-255 (1995-06-01)
We investigated immunohistochemically the localization of lysozyme and Leu M1 in normal skin, 76 cases of benign sweat gland tumors, 28 cases of malignant sweat gland tumors, 23 cases of extramammary Paget's disease, 7 cases of sebaceous carcinoma, 6 cases
G Mazoujian et al.
The American journal of pathology, 110(2), 105-112 (1983-02-01)
Gross cystic disease fluid is a pathologic secretion from breast composed of several glycoproteins, including a unique 15,000-dalton monomer protein, GCDFP-15. By the immunoperoxidase technique, GCDFP-15 was localized in the apocrine metaplastic epithelium lining breast cysts and in apocrine glands
U Raju et al.
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc, 6(5), 516-520 (1993-09-01)
Signet ring carcinoma of the breast often metastasizes to gastrointestinal tract and female genital tract. We report clinicopathologic features of 10 breast carcinomas with signet ring features, five of which had unusual metastatic patterns. The primary breast tumor in all
G Mazoujian et al.
The American Journal of dermatopathology, 10(1), 28-35 (1988-02-01)
Sixty-five cases of benign sweat gland tumors of the skin were studied for the expression and localization of gross cystic disease fluid protein-15 (GCDFP-15) by immunoperoxidase methods. There was positive staining of tumors of probable apocrine differentiation in 10 of
M R Wick et al.
Human pathology, 20(3), 281-287 (1989-03-01)
The identification of metastatic carcinoma of the breast may be difficult in the absence of a previous history of breast cancer. Various immunophenotypic markers have been introduced to aid in this process. A monoclonal antibody directed at a 15-kilodalton (kd)

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