PPB019

Bis-Tris Bicine EDTA buffer

pH 7.2, pHast Pack^™, powder

• Sinonimo/i: 1X Bis-Tris transfer buffer, Bis-Tris
• Codice UNSPSC: 41105319
• NACRES: NA.25

Proprietà

• Nome Commerciale: pHast Pack^™
• Livello qualitativo: 100
• Stato: powder
• Condizioni di stoccaggio: (Tightly closed. Dry)
• pH: 7.2
• Cationi in tracce: Pb: ≤5 ppm
• Attività estranea: DNAse, free; NICKase, free; Protease, free; RNAse, free
• Temperatura di conservazione: room temp

Descrizione

Descrizione generale

Bis-Tris Bicine EDTA buffer or Bis-Tris buffer (pH 7.2) contains Bis-Tris and Bicine, zwitterionic buffer components that form strong bonds with common metals. In combination with ethylenediaminetetraacetic acid (EDTA), a metal chelator, that binds divalent cations. This buffer is commonly used as a transfer buffer in western blot to transfer proteins from the gel to the membrane.

Applicazioni

Bis-Tris buffer may be used in western blot as a transfer buffer.

Caratteristiche e vantaggi

• Ready-to-use buffer
• No measuring and pH adjustment needed
• Certified quality for reproducibility
• Minimizes exposure to toxic chemicals
• Biological tests: free of DNase, RNase, Protease, and Nickase
• Chemical tests: Iron ≤10 ppm, Lead ≤5 ppm

Confezionamento

Foil pouches

Ricostituzione

Contents of one pouch, when dissolved in 500 mL of ultrapure water, will yield a 1X solution containing 25 mM Bicine, 25 mM Bis-Tris, 1 mM EDTA, pH 7.2 at 25 °C. Contents tested to be DNAse, RNAse, Protease, and Nickase free.

Stoccaggio e stabilità

Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.

Altre note

For additional information on our range of Biochemicals, please complete this form.

Note legali

pHast Pack is a trademark of Sigma-Aldrich Co. LLC

Informazioni sulla sicurezza

• Pittogrammi: GHS05
• Avvertenze: Danger
• Indicazioni di pericolo: H318
• Consigli di prudenza: P280 - P305 + P351 + P338
• Classi di pericolo: Eye Dam. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3