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93595

Sigma-Aldrich

Trypan Blue

0.4%, for microscopy

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About This Item

Formula empirica (notazione di Hill):
C34H24N6Na4O14S4
Numero CAS:
72-57-1
Peso molecolare:
960.81
Beilstein:
4360496
Numero MDL:
MFCD00003969
Codice UNSPSC:
12171500
ID PubChem:
329770254
NACRES:
MA.02

Grado

for microscopy

Livello qualitativo

200

Forma fisica

liquid

Durata

limited shelf life, expiry date on the label

Concentrazione

0.4%

Colore

blue to very dark blue

Densità

1.007 g/mL at 20 °C

εmax

1.4 at 603 nm in methanol

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

[Na+].[Na+].[Na+].[Na+].Cc1cc(ccc1N=Nc2c(O)c3c(N)cc(cc3cc2S([O-])(=O)=O)S([O-])(=O)=O)-c4ccc(N=Nc5c(O)c6c(N)cc(cc6cc5S([O-])(=O)=O)S([O-])(=O)=O)c(C)c4

InChI

1S/C34H28N6O14S4.4Na/c1-15-7-17(3-5-25(15)37-39-31-27(57(49,50)51)11-19-9-21(55(43,44)45)13-23(35)29(19)33(31)41)18-4-6-26(16(2)8-18)38-40-32-28(58(52,53)54)12-20-10-22(56(46,47)48)14-24(36)30(20)34(32)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
GLNADSQYFUSGOU-UHFFFAOYSA-J

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Descrizione generale

Trypan Blue is a tetrasulfonated anionic, hydrophilic azo dye derived from toluidine. The non-permeable cell membrane dye is a vital dye that selectively stains dead or non-viable cells, that pass through damaged but not through intact cell membranes.

Applicazioni

Trypan blue applications include:
  • it is a routinely used for viability testing for diverse cell types including frozen sperm and aortic muscle cells exposed to antifungal agents.
  • employed as a collagen stain in a Van Gieson procedure
  • To stain amyloid, Trypan blue is considered as a blue alternative to Congo red in Puchtler-Bennhold stains.
  • used as a fluorescent tracer of cell populations in embryology
  • used as a tumor promoter modulating permeability of lysosomal membranes
Trypan Blue solution has also been used in the cytotoxity tests and to determine the vital cells for the analysis of cell division.

Azioni biochim/fisiol

Trypan Blue is excluded by most living cells, but can be taken into phagocytes and certain other cells.

Stato fisico

0.4% in 0.81% sodium chloride; 0.06% potassium phosphate, dibasic

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H350

Classi di pericolo

Carc. 1B

Codice della classe di stoccaggio

6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

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Certificati d'analisi (COA)

Lot/Batch Number
BCCK2372
BCCJ9155
BCCJ0146
BCCH2945
BCCG5341

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