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Key Documents

910090

Sigma-Aldrich

Fluorescent Brightener 28 disodium salt solution

used as a stain and brightening agent

Sinonimo/i:

Fluostain I, Calcofluor White LRP, Calcofluor White ST, Calcofluor white M2R, Tinopal LPW

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About This Item

Formula condensata:
C40H42N12O10S2Na2
Numero CAS:
Peso molecolare:
960.95
Numero MDL:
Codice UNSPSC:
12171501
NACRES:
NA.47

Forma fisica

liquid

Concentrazione

25% in water

Stringa SMILE

[Na+].[Na+].OCCN(CCO)c1nc(Nc2ccccc2)nc(Nc3ccc(\C=C\c4ccc(Nc5nc(Nc6ccccc6)nc(n5)N(CCO)CCO)cc4S([O-])(=O)=O)c(c3)S([O-])(=O)=O)n1

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Descrizione generale

Fluorescent Brightener 28, also known as Calcofluor White M2R or Fluostain I, is a large, anionic, stilbene derivative of 4,4’-diaminostilbene-2,2’-disulphonic acid. It is a non-specific fluorochrome that binds with cellulose and chitin contained in the cell walls of fungi and other organisms and is used as a stain and fluorescent brightening agent. It exhibits an absorption maximum of 350 nm in methanol and fluoresces blue-white when excited in the violet or near ultraviolet. It bears additional triazinyl and phenyl aromatic groups. The dianionic sulfonate is hydrophilic. It is commercially available as a disodium salt.

Applicazioni

  • Fluorescent Brightener 28 is used in microscopy as fluorochromes, especially to visualize plant tissues by binding to cellulose.
  • It is used in microbiology for staining pathogenic fungi, fungal cell walls, Candida albicans biofilms, for the automated counting of bacteria and spores, and as a viability stain.
  • It is a rapid method for the detection of many yeasts and pathogenic fungi such as Microsporidium, Acanthamoeba, Pneumocystis, Naegleria, and Balamuthia species.
  • It is used industrially as a fluorescent brightening agent for cellulose and polyamide fabrics, paper, detergents, and soaps.
  • It has been used in the identification and study of the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris, and to elucidate the location of chitin in skeletal structures.

Azioni biochim/fisiol

The extended molecule of Calcofluor white M2R binds to cellulose but does not enter living cells, and therefore can be used to visualize plant tissues and viable cells. It absorbs ultraviolet radiation to emit blue light and exhibits enhanced fluorescence when bound to chitin.

Caratteristiche e vantaggi

  • Facilitates rapid detection of many pathogenic yeasts and fungi.
  • As a brightening agent, its addition can offset the tendency of white materials to yellow with age.

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable


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Hermann Ehrlich et al.
Journal of structural biology, 183(3), 474-483 (2013-07-09)
This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques ((13)C solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining);
Jana Klose et al.
Eukaryotic cell, 5(12), 2047-2061 (2006-09-26)
The transition from yeast-like to filamentous growth in the biotrophic fungal phytopathogen Ustilago maydis is a crucial event for pathogenesis. Previously, we showed that fatty acids induce filamentation in U. maydis and that the resulting hyphal cells resemble the infectious

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