NTMIXKB

KAPA dNTP Mix (10 mM each)

• Sinonimo/i: DNTP, PCR
• Codice UNSPSC: 41106300
• NACRES: NA.55

Proprietà

• Grado: Molecular Biology
• Livello qualitativo: 100
• Saggio: >99% (HPLC)
• Forma fisica: liquid
• Durata: ≤18 mo.
• Confezionamento: pkg of 5 mL (KK1041); pkg of 500 μL (KK1017)
• Produttore/marchio commerciale: Roche
• Impurezze: Bacterial contamination assay, passes test; DNase, none detected; Endonuclease contamination assay, passes test; Exonuclease contamination assay, passes test; Human contamination assay, passes test; RNase, none detected; nicking activity, none detected; phosphatase, none detected; protease, none detected
• Colore: colorless
• Solubilità: water: miscible
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

KAPA dinucleotide triphosphates (dNTPs) provide high-quality, molecular, premium grade dinucleotide phosphates (dNTPs) mainly used for polymerase chain reaction (PCR).

Applicazioni

KAPA dNTP Mix has been used in:

• Standard PCR
• High fidelity PCR
• Quantitative PCR
• End-repair or A-tailing
• cDNA synthesis
• DNA sequencing
• DNA labeling
• Microarrays

KAPA dNTPs are suitable for use in any molecular biology application requiring high-quality dNTPs.

Caratteristiche e vantaggi

• KAPA dNTPs are available as individual dNTPs or as a ready-to-use dNTP Mix.
• KAPA dNTP Mix is supplied with KAPA Taq, KAPA2G Fast, KAPA2G Robust, KAPA LongRange and KAPA HiFi PCR Kits.
• KAPA dNTPs are subjected to strict quality control to ensure high performance, particularly with B-family polymerases that are sensitive to poisoning by uracil.
• Compatible with any commercially available DNA polymerases.

Qualità

KAPA dNTPs are >99% pure by HPLC and are free of DNase, RNase, protease, phosphatase, and nicking activity. All kit components are subjected to stringent functional quality control, and meet strict requirements with respect to DNA contamination.

Nota sulla preparazione

Always ensure that the product KAPA dNTP Mix has been fully thawed and mixed before use. KAPA dNTPs may be stored at 4°C for short-term use (up to 1 month). Return to -20°C for long-term storage. Provided that the sample has been handled carefully and not contaminated, the samples are not expected to be compromised if left (unintentionally) at room temperature for a short period of time (up to 3 days). Long-term storage at room temperature and 4°C is not recommended. Please note that storing the reagents at temperatures above -20°C makes them more prone to degradation when contaminated during use, and therefore storage at such temperatures is at the user′s own risk.

Altre note

For Research Use Only. Not for use in diagnostic procedures.

Solo come componenti del kit

• Mixture of dATP, dCTP, dGTP and dTTP 10 mM each

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): nwg
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash