DTTP-RO

dTTP

PCR Grade, sodium salt

• Sinonimo/i: Thymidine 5′-triphosphate sodium salt solution, TTP
• Numero CAS: 18423-43-3
• Numero MDL: MFCD00080338
• Codice UNSPSC: 12352302
• ID PubChem: 329798929

Proprietà

• Livello qualitativo: 100
• Saggio: 99% (dNTP)
• Forma fisica: solution
• impiego: sufficient for 125,000 standard reactions (03732711001); sufficient for 31,250 standard reactions (11969048001); sufficient for 6,250 standard reactions (11934546001)
• Confezionamento: pkg of 1,250 μL (11969048001 [100 mM]); pkg of 4 × 1,250 μL (03732711001 [4x100 mM]); pkg of 250 μL (11934546001 [100 mM])
• Produttore/marchio commerciale: Roche
• Concentrazione: <0.9% (dNDP); 100 mM
• Temperatura di conservazione: −20°C
• Stringa SMILE: [Na].CC1=CN(C2CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O2)C(=O)NC1=O
• InChI: 1S/C10H17N2O14P3.Na.H/c1-5-3-12(10(15)11-9(5)14)8-2-6(13)7(24-8)4-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h3,6-8,13H,2,4H2,1H3,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;; KAELIXTVSANDSE-UHFFFAOYSA-N

Descrizione

Descrizione generale

PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Thymidine 5′-triphosphate sodium salt solution is used in DNA synthesis reactions such as PCR, DNA sequencing, cDNA synthesis and molecular cloning techniques.

dTTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3).

Applicazioni

dTTP, PCR Grade, is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Qualità

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Stato fisico

solution of dTTP in water (pH 8.3).

Nota sulla preparazione

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Altre note

For general laboratory use.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash