SCC048
N27 Rat Dopaminergic Neural Cell Line
Rat
Sinonimo/i:
Dopamine Neural Cells, 1RB3AN27 Cell Line
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About This Item
Codice UNSPSC:
41106514
eCl@ss:
32011203
NACRES:
NA.81
Prodotti consigliati
product name
N27 Rat Dopaminergic Neural Cell Line, The N27 rat dopaminergic neural cell line has been used as a dopaminergic neuron model for in vitro and in vivo studies.
Origine biologica
rat
Livello qualitativo
tecniche
cell culture | stem cell: suitable
Condizioni di spedizione
liquid nitrogen
Descrizione generale
Parkinson’s disease is a neurodegenerative disorder of the central nervous system that affects more than 6 million people worldwide. The motor symptoms of Parkinson′s disease result from the death of dopamine generating cells in the substantia nigra, a region of the midbrain. Numerous efforts have been made to slow the loss or replace these dopamine producing neurons in vivo.
The N27 rat dopaminergic neuron cell line was harvested from E12 rat mesencephalic tissue and was transfected with SV40 to immortalize the cell line. The N27 cell line, when injected into the striata of 6-hydroxydopamine-lesioned rats (an animal model of PD) caused a time-dependent improvement in neurological deficits. This immortalized cell line has been carefully characterized in studies of dopamine biosynthesis, neurotoxicity and used as a dopaminergic neuron model for in vitro and in vivo studies.
Descrizione della linea cellulare
Neural Stem Cells
Neural Lineage Cells
Neural Lineage Cells
Applicazioni
Research Category
Stem Cell Research
Neuroscience
Stem Cell Research
Neuroscience
Research Sub Category
Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
Neural Stem Cells
Neurochemistry & Neurotrophins
Neurodegenerative Diseases
Neuroregenerative Medicine
This product is intended for sale and sold solely for internal non-commercial research use per the terms of the “Restricted Use Agreement” as detailed in the product documentation. For information regarding any other uses, please contact licensing@emdmillipore.com.
Qualità
• Each vial contains ≥ 1X106 viable cells.
• Cells are negative for mycoplasma contamination.
• Cells are negative for mycoplasma contamination.
Stoccaggio e stabilità
N27 cells should be stored in liquid nitrogen. The cells can be passage for at least 10 passages without significantly affecting the cell marker expression and functionality.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 1
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Establishment and characterization of immortalized clonal cell lines from fetal rat mesencephalic tissue.
Prasad, K N, et al.
In Vitro Cellular & Developmental Biology. Animal, 30A, 596-603 (1994)
Improvement of neurological deficits in 6-hydroxydopamine-lesioned rats after transplantation with allogeneic simian virus 40 large tumor antigen gene-induced immortalized dopamine cells.
Clarkson, E D, et al.
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