RADPCMAG-82K
MILLIPLEX® Rat Adipocyte Panel - Metabolism Assay
About This Item
Prodotti consigliati
Reattività contro le specie
rat
Livello qualitativo
Produttore/marchio commerciale
Milliplex®
assay range
accuracy: 94-121%
sensitivity: 0.1-25 pg/mL
(MinDC+2SD)
standard curve range: 0.7-3,000 pg/mL
(IL-1β, TNFα)
standard curve range: 12-50,000 pg/mL
(PAI-1)
standard curve range: 122-500,000 pg/mL
(Adiponectin)
standard curve range: 15-60,000 pg/mL
(IL-6, Leptin)
standard curve range: 9.8-40,000 pg/mL
(MCP-1)
tecniche
multiplexing: suitable
Metodo di rivelazione
fluorometric (Luminex xMAP)
Condizioni di spedizione
wet ice
Descrizione generale
The MILLIPLEX® Rat Adipocyte Panel is a 7-plex kit to be used for the simultaneous quantification of any or all of the following analytes in tissue/cell lysates and culture supernatant samples: Adiponectin, IL-1β, IL-6, Leptin, MCP-1, PAI-1 Total, and TNFα. This kit uses a 96-well format, contains a lyophilized standard cocktail, two internal assay quality controls and can measure up to 38 samples in duplicate.
The Luminex® xMAP® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.
Panel Type: Metabolism
Specificità
There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Applicazioni
- Analytes: Adiponectin, IL-1β, IL-6, Leptin, MCP-1 (CCL2), PAI-1 (Total), TNFα
- Recommended Sample Type: Rat cell/tissue culture supernatant or lysates.
- Recommended Sample Dilution: 25 μL per well of cell/Tissue culture sample. Samples may require a dilution with an appropriate control medium.
- Assay Run Time: Overnight (16-18 hours) at 2-8°C
- Research Category: Metabolism
- Research Subcategory: Endocrine; Obesity; Metabolic Disease
Caratteristiche e vantaggi
Confezionamento
Componenti
Rat Adipokine Quality Control 1, 1 vial, lyophilized
Rat Adipokine Quality Control 2, 1 vial, lyophilized
Assay Buffer, 1 bottle, 30 mL
Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea
Rat Adipocyte Detection Antibodies, 1 bottle, 5.5 mL
Streptavidin-Phycoerythrin, 1 bottle, 5.5 mL
Mixing Bottle, 1 bottle
Sample Plate, 96 well format, 1 plate
2 Foil Plate Sealers
Stoccaggio e stabilità
Altre note
Note legali
Esclusione di responsabilità
Avvertenze
Danger
Indicazioni di pericolo
Classi di pericolo
Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1
Codice della classe di stoccaggio
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Certificati d'analisi (COA)
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Contenuto correlato
Multiplex immunoassays, such as MILLIPLEX® multiplex metabolic assays, are critical in metabolic syndrome research because they provide a full picture of the different conditions related to it, like diabetes and obesity, as well as save time and sample volume.
Learn the benefits of using biomarker detection multiplex immunoassays for laboratory, companion, and agricultural animal research and explore MILLIPLEX® multiplex assays for veterinary medicine and animal health research.
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